In vitro study on the effect of ribavirin reducing the EV71 replication / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>Observe the effect of Ribavirin on reducing the EV71 replication, inactivating EV71 and protecting the RD-A cells against the EV71 infection in vitro.</p><p><b>METHODS</b>Using the EV71 isolated from Anhui Fuyang HFMD outbreak, the effect of Ribavirin on RD-A cells during the EV71 infection was observed.</p><p><b>RESULTS</b>In the experiment of Ribavirin inhibiting the EV71 replication, comparing with the no-Ribavirin-dealed virus control group, the group of 1:640 dilution of Ribavirin delayed the CPE for 2 days, while the normal cell group was growing very well. In the experiment of protecting cell from EV71 infection, comparing with the no-Ribavirin-dealed virus control group, the group of 1:8 dilution of Ribavirin delayed the CPE for 4 days. In the experiment of Ribavirin effect on the inactivation of EV71, the group of 1:40 dilution of Ribavirin delayed the CPE for 2 days comparing with the virus control group.</p><p><b>CONCLUSION</b>Ribavirin could inhibit the replication of the EV71 and inactivate the EV71 in vitro. Additionally, Ribavirin could protect RD-A cells from EV 71 infection. The observation will contribute to EV71 infection control and quick medicine therapy.</p>

Seroepidemical study of Coxsackievirus A 16, in four provinces, China, 2005 / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To study the situation of 1- 5-years-old children's antibody against Coxsackievirus A group 16 strain (CVA16) in Guangdong, Heilongjiang,Yunnan Province and Xinjiang Uygur Autonomous Regions, China, 2005, it can offer scientific evidences for preventing and controlling CVA16 causative hand-food and mouth disease.</p><p><b>METHODS</b>Using microneutrilization test, to study 503 serum samples randomly selected from sera collected in 2005.</p><p><b>RESULTS</b>Positive rate of anti-CVA16 antibody were 41.90%, 9.40%, 40.00% and 34.40% in Guangdong, Heilongjiang,Yunnan and Xinjiang, respectively. Antibody titer was relative low (average, 1: 6.1) and there was no statistical difference of geometry mean of antibody titer (GMT) among Guangdong, Heilongjiang, Yunnan (F = 0.97, 0.40, 1.06, respectively; P > 0.05), while there had statistical difference of GMT between Heilongjiang and other three regions( F = 10.61, P < 0.00).</p><p><b>CONCLUSIONS</b>There had probably existed local epidemic in some regions of Guangdong, Heilongjiang, Yunnan Province and Xinjiang Uygur Autonomous Regions, China, 2005 or even before, but the area and degree of transmission and epidemic had difference. Children aged from 1- 5-years-old were relatively susceptible population of CVA16 infection.</p>